PARTNER 18-1 – UHEI-KIP

 

Address:
Kirchhoff Institut für Physik
Im Neuenheimer Feld 227
D-69120 Heidelberg
Germany

Telephone:  ++49(6221)549252

Fax:  ++49(6221)549112

Email:  cremer@kip.uni-heidelberg.de

Webpage:   http://www.kip.uni-heidelberg.de/AG_Cremer/

 

Members

Name

Email

Telephone

Prof. Dr. Christoph Cremer

cremer@kip.uni-heidelberg.de

++49(6221)549252

Dr. Udo Spöri

uspoeri@kip.uni-heidelberg.de

++49(6221)549274

Jürgen Reymann

jrey@kip.uni-heidelberg.de

++49(6221)549274

Roman Amberger

amberger@kip.uni-heidelberg.de

++49(6221)549253

Werner Stadter

wstadter@kip.uni-heidelberg.de

++49(6221)549274

 

Main Research Activities

The main topic of our research is biophysics of mammalian nuclear genome structure. Present research activities include

  • Instrumentation development in far field light microscopy such as Spectral Precision Distance Microscopy (SPDM) Spatially Modulated Illumination (SMI) Microscopy, Micro-axial Tomography
  • Topology and size analyses of cellular nanostructures
  • modelling of the dynamic structure of specific gene regions and of whole chromosome territories

 

Most Relevant Publications

(1)  H. Bornfleth, P. Edelmann, D. Zink, T. Cremer, C. Cremer (1999): Quantitative motion analysis of sub-chromosomal foci in vivo in four-dimensional microscopic images. Bio­phys. Journal 77: 2871 – 2886.

 

(2)    H. Tanabe, S. Müller, M. Neusser, J.v. Hase, E, Calcagno, M. Cremer, I. Solovei, C. Cremer, T. Cremer (2002): Evolutionary conservation of chromosome territory arrangements in cell nuclei from higher primates. Proc. Natl. Acad. Sci. USA 99: 4424 – 4429

 

(3)  A. Esa, P. Edelmann, L. Trakhtenbrot, N. Amariglio, G. Rechavi, M. Hausmann, C. Cremer (2000) 3D-spectral precision distance microscopy (SPDM) of chromatin nanostructures after triple-colour labeling: a study of the BCR region on chromosome 22 and the Philadelphia chromosome. J. Microscopy 199: 96 – 105.

 

(4)  T. Cremer & C. Cremer (2001) Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nature Reviews Genetics 2: 292 – 301.

 

(5)  B. Albrecht, A. Schweitzer, A.V. Failla, P. Edelmann, C. Cremer (2002): Spatially modulated illumination (SMI) microscopy allows axial distance resolution in the nanometer range. Applied Optics 41, 80 - 87.

(6)  A. V. Failla, U. Spoeri, B. Albrecht, A. Kroll, C. Cremer (2002b) Nanosizing of fluorescent objects by spatially modulated illumination microscopy. Appl. Optics 41: 7275 – 7283.

 

(7)  S. Martin, A. V. Failla, U. Spöri, C. Cremer, A. Pombo (2004) Measuring the Size of Biological Nanostructures with Spatially Modulated Illumination Microscopy. Mol. Biol. Cell, 15, 2449-2455.