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PARTNER 16-2 – EMCR |
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Address: Department of Cell Biology and Genetics 3000 DR The |
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Telephone: 31 10 408 8337 |
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Fax: 31 10 408 9468 |
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Email: c.wyman@erasmusmc.nl |
Members
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Name |
Email |
Telephone |
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Claire
Wyman |
31
10 408 8337 |
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Kris
Leslie |
31
10 408 8338 |
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Main
Research Activities Nano scale imaging of DNA protein complexes involved in genome
metabolism by scanning force microscopy (SFM also called AFM). Current topics
in genome metabolism include DNA repair and chromatin remodeling. Methods to
tag proteins with nano opjects such as gold spheres are being developed for
imporved contrast in SFM imaging (SP I).
We are defining nanoscale architecture and interactions of protien
complexes involved in DNA repair and chromatin remodeling. This infomation will be used to try and
detect similar nanoscale structures and interactions in cells and organisms
(SP III). |
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Most Relevant
Publications Beerens, N., J.H.J. Hoeijmakers, R. Kanaar, W.
Vermeulen, and C. Wyman. (2005) The CSB protein actively wraps DNA. J. Biol. Chem. 280, p.
4722-4729. http://www.jbc.org/cgi/content/full/280/6/4722 Wyman,
C., D. Ristic and R. Kanaar. (2004) Homologous recombination-mediated double-strand break
repair. DNA Repair, 3, p.
827-33. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6X17-4C7J836-1&_cover Janicijevic, A., D. Ristic and C. Wyman. (2003) The
molecular machines of DNA repair: Scanning force microscopy analysis of their
architecture. Journal of Microscopy.
212, p. 264-272 http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm |